Composite

Part:BBa_K4147004:Design

Designed by: César Ibrahym Rodríguez Fernández   Group: iGEM22_Tec-Chihuahua   (2022-09-30)


DrsB1 Construct with LacI Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When sending the part to synthetize, be sure to add some extra bases at the ends of the prefix and suffix, otherwise, the restriction enzymes might not be able to digest, making it difficult to later ligate in a vector.


Source

The original sequence was taken from the iGEM Part Registry, as well as the added parts for expression. The MrsA2 CDS was added by team iGEM18_WPI_Worcester (https://parts.igem.org/Part:BBa_K2577001)

References